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AssigneeAgilent Technologies, Inc.
InventorsJoseph A. Sorge, Reinhold Dietrich Mueller, Gothami Padmabandu, Nick Roelofs, Holly H. Hogrefe
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The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3′-5′ exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3′-5′ exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with deficient 3′-5′ exonuclease activity and reduced base analog detection activity.
An Archaeal DNA polymerase comprising at least one amino acid mutation in the exoI motif and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:
An Archaeal DNA polymerase comprising at least one amino acid mutation in the exoII motif and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:83-88 or SEQ ID NOs:90-102, wherein said Archaeal DNA polymerase is deficient in 3′-5′ exonuclease activity.
An Archaeal DNA polymerase comprising at least one amino acid mutation in the exo III motif and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:83-88 or SEQ ID NOs:90-102, wherein said Archaeal DNA polymerase is deficient in 3′-5′ exonuclease activity.
An Archaeal DNA polymerase comprising at least one amino acid mutation in each of the exo I and exo III motifs and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:83-88 or SEQ ID NOs:90-102, wherein said Archaeal DNA polymerase is deficient in 3′-5′ exonuclease activity.
An Archaeal DNA polymerase comprising at least one amino acid mutation in each of the exo II and exo III motifs and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:83-88 or SEQ ID NOs:90-102, wherein said Archaeal DNA polymerase is deficient in 3′-5′ exonuclease activity.
An Archaeal DNA polymerase comprising at least one amino acid mutation in each of the exo I and exoII motifs and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:83-88 or SEQ ID NOs:90-102, wherein said Archaeal DNA polymerase is deficient in 3′-5′ exonuclease activity.
An Archaeal DNA polymerase comprising at least one amino acid mutation in each of the exoI, exo II, and exoIII motifs and an amino acid mutation at V93 of SEQ ID NO:89 or a corresponding residue in an amino acid sequence selected from SEQ ID NOs:
Dependencies are inferred from the claim text.