Processing enzymes fused to basic protein tags
AssigneeNovo Nordisk Health Care AG
InventorAllan Christian Shaw
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Abstract
The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.
Claims19
A processing enzyme comprising an N-terminally attached tag derived from a ribosomal protein from a thermophilic bacteria, wherein at least 15% of the protein's amino acids comprise Lys, Arg or a combination thereof, wherein the tag is attached to the processing enzyme by a linker sequence comprising a sequence selected from the group consisting of SEQ ID NO:
- 29, SEQ ID NO:
- 30, SEQ ID NO:
- 31, SEQ ID NO:
- 32, SEQ ID NO:33, and SEQ ID NO:
- 34.
Dependencies are inferred from the claim text.