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US 8,283,143 B2Granted
Enhanced production of fatty acid derivatives
AssigneeLS9, Inc.
InventorsZhihao Hu, Fernando Valle
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Abstract
Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use.
Claims16
A method of producing a fatty alcohol composition, which method comprises the steps of:
- (a) obtaining a genetically engineered microbial cell comprising polynucleotide sequences encoding polypeptides having (i) acetyl-CoA carboxylase (accABCD, EC 6.4.1.2) activity and (ii) fatty alcohol forming activity selected from the group consisting of alcohol forming acyl-CoA reductase (FAR, EC 1.1.1.*), acyl-CoA reductase (EC 1.2.1.50) and alcohol dehydrogenase (EC 1.1.1.1),
- (b) culturing the genetically engineered cell in a culture medium containing a carbon source under conditions effective to overexpress the polynucleotide sequences encoding the polypeptides having accABCD activity and fatty alcohol forming activity,
- (c) producing a fatty alcohol in the genetically engineered microbial cell, and
- (d) optionally recovering the fatty alcohol composition from the cell culture medium.
1 more element
Dependencies are inferred from the claim text.