Back to results
US 8,283,142 B2Granted
Stabilization of perhydrolases
AssigneeE. I. Du Pont de Nemours and Company
InventorsRobert Dicosimo, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
Claims17
A process for enzymatically producing a peroxycarboxylic acid comprising:
- (a) providing a set of reaction components, said components comprising:
- (1) a formulation comprising a mixture of:
- (I) at least one carboxylic acid ester selected from the group consisting of monoacetin, diacetin, triacetin, monopropionin, dipropionin, tripropionin, monobutyrin, dibutyrin, tributyrin, and mixtures thereof;
- (II) an enzyme powder comprising
- (i) at least one enzyme structurally classified as a CE-7 enzyme and having perhydrolvsis activity and a signature motif comprising:
- (A) an RGQ motif at amino acid residues 118-120,
- (B) a GXSQG motif at amino acid residues 179-183, and
- (C) an HE motif at amino acid residues 298-299 when aligned to reference sequence SEQ ID NO:1 using CLUSTALW,
- (ii) at least one excipient, and
- (iii) optionally at least one surfactant;
- and
- (III) at least one buffer;
- wherein said at least one buffer enhances the stability of said at least one enzyme when present in said formulation as compared to the perhydrolase activity of said at least one enzyme when the at least one excipient is not present;
- and
- (2) a source of peroxygen in water;
- and
- (b) combining said reaction components whereby a peroxycarboxylic acid is produced.
14 more elements
Dependencies are inferred from the claim text.