Method for the high level expression of active tumor necrosis receptor family member immunoglobulin fusion proteins

A method for increasing the expression of active TNF receptor family member-Ig Fc fusion proteins and minimizing expression of inactive TNF receptor family member-Ig Fc fusion proteins comprising culturing a mammalian host cell transformed with DNA molecule encoding a desired TNF receptor-Ig fusion protein in a mammalian cell culture having a minimum temperature of about 27° C. to a maximum temperature of less than or equal to 32° C. and recovering the active TNF receptor family member-Ig Fc fusion proteins from the culture, wherein the transformed host cell is first cultured at a temperature of about 33° C. to about 37° C. for a period of time sufficient to allow growth of said host cell, and wherein the active TNF receptor family member is involved in immune regulation and comprises 2-4 copies of canonical TNF family cysteine-rich domains.

A method for increasing expression of active LTβR-Ig Fc fusion proteins and minimizing expression of inactive LTβR-Ig Fc fusion proteins comprising culturing a mammalian host cell transformed with a DNA molecule encoding an LTβR-Ig fusion protein in cell culture having a minimum temperature of about 27° C. and a maximum temperature of less than or equal to 32° C. and isolating the active LTβR-Ig Fc fusion proteins from the culture medium using hydrophobic interaction chromatography, wherein the transformed host cell is first cultured at a temperature of about 33° C. to about 37° C. for a period of time sufficient to allow growth of said host cell.