Process for producing recombinant fibrinogen highly producing cell and highly producing cell
AssigneeJuridical Foundation The Chemo-Sero-Therapeutic Research Institute
InventorsReiko Matsuyama, Hiroaki Maeda
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Abstract
When genes encoding three kinds of proteins constituting fibrinogen, an α chain (or variant of α chain), a β chain and a γ chain (or variant of γ chain) are incorporated into an animal cell, a constitutional ratio of respective genes is such that a α chain (and/or variant of γ chain) gene is an equal amount to a 1000-fold amount relative to an α chain (and/or variant of α chain) gene and a β chain gene and, further, by using a baculovirus P35 gene, a recombinant fibrinogen highly producing cell is prepared.
Claims16
A process for preparing a recombinant fibrinogen-producing cell which produces a high level of fibrinogen of 100 μg/ml or more, comprising transfecting, into an animal cell, genes encoding (i) an α chain and/or an αE variant thereof, (ii) a β chain, and (iii) a γ chain and/or a γ′ variant thereof which are polypeptides constituting fibrinogen, wherein the number of genes encoding a γ chain and/or a γ′ variant thereof is 1 to 3 times the sum of the number of genes encoding an α chain and/or an αE variant thereof and the number of genes encoding a β chain, wherein the animal cell transfected with the genes encoding the chains produces a high level of fibrinogen of 100 μg/ml or more.
Dependencies are inferred from the claim text.