Protein fragments of virB10 and sero-detection of anaplasma phagocytophium
AssigneeMedical Diagnostic Laboratories, LLC
InventorsJohn G. Hoey, Denise P. Dimitrov, Lisa P. Huang, Martin E. Adelson, Eli Mordechai
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Abstract
Disclosed are cloning and expression of a plurality of protein fragments of virB10, a Type IV Secretion System (TIVSS) in Anaplasma phagocytophilum. Such recombinant protein fragments are useful in the ELISA detection of anaplasma pathogen. The use of same as kits for ELISA is also disclosed.
Claims13
A method of detecting the presence of an antibody against Anaplasma phagocytophilum in a biological sample of a mammal comprising:
- (i) immobilizing an isolated polypeptide antigen onto a surface, wherein said immobilized isolated polypeptide antigen has the amino acid sequence set forth in SEQ ID NO:
- 13 or SEQ ID NO:
- 15;
- (ii) contacting said immobilized isolated polypeptide antigen with the biological sample of the mammal, under conditions that allow formation of an antibody-antigen complex, said biological sample containing an antibody against Anaplasma phagocytophilum, wherein said antibody binds to said immobilized isolated polypeptide antigen and forms a complex of the antibody and the polypeptide antigen;
- and
- (iii) detecting the formation of said antibody-polypeptide antigen complex, wherein said detected antibody-polypeptide antigen complex is indicative of the presence of said antibody against Anaplasma phagocytophilum in said biological sample.
A method of diagnosing an infection of Anaplasma phagocytophilum in a mammal by detecting the presence of an antibody against said Anaplasma phagocytophilum comprising the steps of:
- (i) obtaining a biological sample from the mammal, wherein said mammal is suspected of having the Anaplasma phagocytophilum infection;
- (ii) immobilizing an isolated polypeptide antigen onto a surface, wherein said immobilized isolated polypeptide antigen has the amino acid sequence set forth in SEQ ID NO:
- 13 or SEQ ID NO:
- 15;
- (iii) contacting said immobilized isolated polypeptide antigen with said biological sample, under conditions that allow formation of an antibody-antigen complex, said biological sample containing an antibody against Anaplasma phagocytophilum, and wherein said antibody binds to said immobilized isolated polypeptide antigen and forms a complex of the antibody and the polypeptide antigen;
- and
- (iv) detecting said antibody-polypeptide antigen complex, wherein said detected antibody-polypeptide antigen complex is indicative of the presence of said antibody against Anaplasma phagocytophilum in said biological sample.
Dependencies are inferred from the claim text.