Voltage sensor domains of voltage-dependent ion channel proteins and uses thereof
AssigneeThe Rockefeller University
InventorsRoderick MacKinnon, Alice Lee MacKinnon, Youxing Jiang, Vanessa Ruta
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Abstract
A composition of matter suitable for use in identifying chemical compounds that bind to voltage-dependent ion channel proteins, the composition comprising a screening protein that comprises an ion channel voltage sensor domain of the ion channel protein immobilized on a solid support.
Claims22
A composition of matter suitable for use in identifying chemical compounds that bind to a voltage-dependent sodium ion channel protein, the composition comprising a screening protein consisting essentially of a sodium ion channel voltage sensor domain having transmembrane segments S1-S4 of the ion channel protein immobilized on a solid support.
A kit suitable for use in identifying chemical compounds that bind to a voltage-dependent ion channel protein, the kit comprising a screening protein consisting essentially of a sodium ion channel voltage sensor domain having transmembrane segments S1-S4 of the ion channel protein and a solid support.
A labeled screening protein suitable for use in identifying chemical compounds that bind to a voltage-dependent sodium ion channel protein, the labeled screening protein consisting essentially of a sodium ion channel voltage sensor domain having transmembrane segments S1-S4 of the ion channel protein and a detectable label.
A method for screening for drug candidates that target voltage dependent sodium ion channel protein, the method comprising:
- providing a screening protein consisting essentially of a sodium ion channel voltage sensor domain having transmembrane segments S1-S4 of the ion channel protein;
- contacting the screening protein with a chemical compound;
- determining whether the chemical compound binds to the screening protein;
- wherein chemical compounds that bind to the screening protein are drug candidates.
Dependencies are inferred from the claim text.