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US 8,283,125 B2Granted
Peptide and protein preservation method
AssigneeBiomedal, S.L.
InventorsAngel Cebolla Ramirez, Moisés Alvarez Maqueda
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Abstract
The invention relates to a method for preserving peptides or proteins over long periods of time. The invention also relates to the use of the preservation method for the transport of labeled peptides or proteins without requiring refrigeration, as well as to a set of tools for implementing said method.
Claims15
A method for preserving or storing tagged peptides or proteins by means of their reversible binding to solid or semi-solid derivatized matrices, said method comprising:
- (a) passing a fluid or solution that contains the tagged peptide or protein through a solid or semi-solid porous matrix having functional groups, wherein the tag of the peptide or protein binds by affinity to functional groups that are present in the matrix,
- (b) passing a cleaning solution through the matrix to eliminate biological material, peptides or proteins, or other substances of the fluid or solution not bound to the solid or semi-solid derivatized, porous matrix,
- (c) partially or totally drying the matrix comprising the tagged peptide or protein at moderate temperature from 12° to 60° C.
- (d) placing the partially or totally dried matrix comprising the tagged peptide or protein inside a wrapping that is clean and septic during the time required prior to its user, and
- (e) recovering the tagged peptide or protein from the matrix by passing a solution through the matrix that comprises specific compounds that interrupt the affinity binding between the peptide or protein tag and the functional groups of the solid or semi-solid porous matrix.
2 more elements
Dependencies are inferred from the claim text.