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US 8,282,921 B2Granted
tRNA synthetase fragments
OwnerPaul Gliddeninventor
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OwnerPaul Gliddeninventor
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The present invention relates to compositions and methods for treating conditions associated with angiogenesis. In particular the present invention relates to multi-unit complexes of tRNA synthetase fragments and uses thereof; diverse multi-unit complexes including a tRNA synthetase fragment; compositions and methods for modulating angiogenesis; polynucleotides encoding tRNA synthetase fragments and uses thereof; antibodies and epitopes specific to tRNA synthetase fragments; variants of tRNA synthetase fragments and uses thereof; methods for treating angiogenesis; methods for screening for anti-angiogenic agents; methods of modulating angiogenesis; kits for modulating angiogenesis; and business methods for modulating angiogenesis. Preferably the tRNA synthetase fragments are tryptophanyl tRNA synthetase fragments, and more preferably human tryptophanyl tRNA synthetase fragments.
A method for purifying a multiunit complex comprising a first tryptophanyl tRNA synthetase fragment and a second tryptophanyl tRNA synthetase fragment, wherein the purified complex comprises less than 10 endotoxin units per milligram of synthetase fragment, and wherein the method comprises performing an endotoxin-reduction filtration step after performing a clarification step and prior to performing at least one of the steps selected from:
A method for purifying a multiunit complex comprising a first tryptophanyl tRNA synthetase fragment and a second tryptophanyl tRNA synthetase fragment, wherein the purified complex comprises less than 10 endotoxin units per milligram of synthetase fragment, and wherein the method comprises performing an endotoxin-reduction filtration step after performing an anion-exchange chromatographic step, wherein said endotoxin-reduction filtration step does not include the use of a detergent.
Dependencies are inferred from the claim text.