Hydrolysis-resistant polyacrylamide gels
AssigneeBio-Rad Laboratories, Inc.
InventorsCraig Rowell, Cory Panattoni, Thomas R. Berkelman, Sean Cater, Shane Petersen, Lee Olech, Xuemei Yang
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Abstract
Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Claims54
A polyacrylamide gel comprising crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane and being devoid of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
A polyacrylamide gel comprising crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel having about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)methane and being devoid of tris(hydroxymethyl)aminomethane.
A polyacrylamide gel comprising crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)-aminomethane and about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
A method for separating a mixture of proteins by electrophoresis, said method comprising (i) loading a gel with a sample of said mixture wherein said gel comprises crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane and being devoid of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and (ii) imposing a voltage across said gel to cause said proteins to migrate through said gel at differential rates until separated into bands within said gel.
A method for separating a mixture of proteins by electrophoresis, said method comprising (i) loading a gel with a sample of said mixture wherein said gel comprises crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel having about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)aminomethane and being devoid of tris(hydroxymethyl)methane, and (ii) imposing a voltage across said gel to cause said proteins to migrate through said gel at differential rates until separated into bands within said gel.
A method for separating a mixture of proteins by electrophoresis, said method comprising (i) loading a gel with a sample of said mixture wherein said gel comprises crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane and about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and (ii) imposing a voltage across said gel to cause said proteins to migrate through said gel at differential rates until separated into bands within said gel.
Dependencies are inferred from the claim text.