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US 8,278,072 B1Granted
Method for synthesis of sialylated products using reversible sialylation
AssigneesHealth Research, Inc., The Research Foundation of State University of New York
InventorsKhushi L. Matta, Edaya V. Chandrasekaran, Sriram Neelamegham, Jun Xue
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Abstract
A method for producing sialylated molecules based on reverse sialylation that catalytically transfers the sialic acid moiety of sialylated donors to nucleotide monophosphates or transfer sialic acid moieties from sialylated donors to acceptor glycoproteins or glycolipids.
Claims12
A method for sialylating an acceptor glycoconjugate comprising the steps of admixing in a vessel the following components to form a reaction mixture:
- a) a sialic acid donor other than a sialic acid-5′-nucleotide monophosphate (NMP);
- b) an acceptor glycoconjugate;
- and
- c) catalytic amount of exogenous mammalian ST3Gal-II sialyltransferase, under conditions such that a sialylated acceptor glycoconjugate is formed in the reaction mixture.
1 more element
Dependencies are inferred from the claim text.