System for differentiating the lengths of nucleic acids of interest in a sample

A system for differentiating the lengths of nucleic acids of interest in a sample, the system comprising:

• a microfluidic device comprising an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acids of interest in the sample;
• a detector integral with or proximal to the microfluidic device, which detector is configured to detect the one or more amplicons as one or more signals from a homogenous mixture of reaction mixture constituents flowing through the microfluidic device;
• and
• a software system that interprets two or more coincidentally detected signals from the homogenous mixture to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.