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US 8,274,650 B2Granted
Signal noise reduction for imaging in biological analysis
AssigneeApplied Biosystems, LLC
InventorsAustin B. Tomaney, Mark F. Oldham
Life of this patent
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Abstract
A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.
Claims18
A method for a biological system, comprising:
- providing a photodetector comprising a plurality of pixels capable of forming an optical image of fluorescent light emitted from a biological sample;
- exposing the photodetector to a test signal from a biological sample;
- providing pixel noise data for a plurality of pixels of the photodetector;
- generating a response of the plurality of pixels to a uniform input signal;
- based on the response, determining corrected signals data for at least some of the pixels from the plurality of pixels.
2 more elements
An imaging system for analysis of a biological sample, comprising:
- a sample region configured to contain a plurality of biological samples comprising a labeled fragment;
- a photodetector comprising a plurality of pixels;
- optics configured to transfer energy emission from the biological samples to the photodetector;
- a data structure containing pixel noise data for at least some of the plurality of pixels and corrected signals data, the corrected signals data comprising a comparison of a response of the plurality of pixels to a uniform input;
- and
- a processor configured to adjust measurement signals produced by the photodetector based at least in part on the pixel noise data and the corrected signals data.
3 more elements
An imaging system for analysis of a biological sample, comprising:
- a photodetector comprising a plurality of pixels;
- a data structure containing pixel noise data for at least some of the plurality of pixels and corrected signals data, the corrected signals data comprising a comparison of a response of the plurality of pixels to a uniform input;
- and
- a processor configured to adjust measurement signals produced by the photodetector based at least in part on the pixel noise data and the corrected signals data;
- wherein the data structure further comprises one or more of:
- an average of the corrected signals data;
- or
- a normalization factor for at least some of the pixels of the plurality of pixels, the normalization factor being based at least in part on the average.
5 more elements
Dependencies are inferred from the claim text.