Corn event PV-ZMGT32(nk603) and compositions and methods for detection thereof
AssigneeMonsanto Technology LLC
InventorsCarl Frederick Behr, Gregory R. Heck, Catherine Hironaka Hironaka, Jinsong You
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Abstract
The present invention provides a DNA construct that confers tolerance to transgenic corn plant. Also provided are assays for detecting the presence of the PV-ZMGT32(nk603) corn event based on the DNA sequence of the recombinant construct inserted into the corn genome and of genomic sequences flanking the insertion site.
Claims6
A corn plant comprising
- (a) a DNA construct of comprising:
- a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises (i) a rice actin 1 promoter;
- (ii) a rice actin 1 intron;
- (iii) a chloroplast transit peptide encoding DNA molecule;
- (iv) a glyphosate tolerant EPSPS encoding DNA molecule;
- and (v) a transcriptional terminator DNA molecule;
- and said second expression cassette comprising in operable linkage (a) a CaMV 35S promoter;
- (b) a Hsp70 intron;
- (c) a chloroplast transit peptide encoding DNA molecule;
- (d) a glyphosate tolerant EPSPS encoding DNA molecule;
- and (e) a transcriptional terminator DNA molecule;
- and
- (b) a DNA molecule comprising at least two sequences selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12.
A method of producing a corn plant that tolerates application of glyphosate herbicide comprising:
- (a) transforming a corn cell with a DNA construct comprising a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises (i) a rice actin 1 promoter;
- (ii) a rice actin 1 intron;
- (iii) a chloroplast transit peptide encoding DNA molecule;
- (iv) a glyphosate tolerant EPSPS encoding DNA molecule;
- and (v) a transcriptional terminator DNA molecule;
- and said second expression cassette comprising in operable linkage (a) a CaMV 35S promoter;
- (b) a Hsp70 intron;
- (c) a chloroplast transit peptide encoding DNA molecule;
- (d) a glyphosate tolerant EPSPS encoding DNA molecule;
- and (e) a transcriptional terminator DNA molecule;
- (b) selecting said corn cell with tolerance to application of glyphosate, the cell comprising a DNA molecule comprising at least two sequences selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12;
- (c) growing said corn cell into a fertile corn plant.
A corn plant, seed, nucleus, or part thereof capable of producing a PV-ZMGT32(nk603) diagnostic amplicon.
A corn plant, seed, nucleus, or part thereof comprising corn event PV-ZMGT32(nk603).
A glyphosate tolerant corn plant, or DNA-containing part thereof, comprising incorporated into the plant's genome a DNA insert encoding EPSPS from two copies of an EPSPS-encoding gene, wherein the sequence overlapping the junction between the corn genomic DNA and the 5′ end of the insert comprises SEQ ID NO:7 and the sequence overlapping the junction between the corn genomic DNA and the 3′ end of the insert comprises SEQ ID NO:8.
Dependencies are inferred from the claim text.