Compositions and methods for improved glycoprotein sialylation
AssigneeSigma-Aldrich Co.
InventorsMin Zhang, James S. Ross
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions and methods for the production of glycoproteins with enhanced sialylation. In particular, the invention provides cell lines comprising disrupted sialidase expression and methods of using the cell lines to produce glycoproteins with enhanced sialylation.
Claims32
A cell line, the cell line comprising disrupted expression of at least one chromosomally integrated nucleic acid sequence encoding a non-cytoplasmic sialidase, the amino acid sequence of the non-cytoplasmic sialidase is (a) at least about 95% identical to SEQ ID NO:2, and/or (b) at least about 80% identical to SEQ ID NO:4.
A method for producing a glycoprotein, the method comprising expressing a nucleic acid sequence encoding the glycoprotein in a cell comprising disrupted expression of at least one chromosomally integrated nucleic acid sequence encoding a non-cytoplasmic sialidase, the amino acid sequence of the non-cytoplasmic sialidase is (a) at least about 95% identical to SEQ ID NO:2, and/or (b) at least about 80% identical to SEQ ID NO:4, wherein the glycoprotein has increased sialylation relative to a suitable control.
An isolated nucleic acid encoding a polypeptide whose amino acid sequence consists of SEQ ID NO:2 or SEQ ID NO:4.
Dependencies are inferred from the claim text.