Process for purifying proteins
AssigneeAmgen Inc.
InventorsAnna Senczuk, Ralph Klinke
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Abstract
The invention relates to a process for purifying a protein by mixing a protein preparation with a solution having a first salt and a second salt, wherein each salt has a different lyotropic value, and loading the mixture onto a hydrophobic interaction chromatography column. The dynamic capacity of the column for a protein using the two salt combination will be increased compared with the dynamic capacity of the column for either single salt alone.
Claims13
A process for purifying a protein on a hydrophobic interaction chromatography column such that the dynamic capacity of the column is increased for the protein comprising mixing a preparation containing the protein with a combination of a first salt and a second salt, loading the mixture onto a hydrophobic interaction chromatography column, and eluting the protein, wherein the first and second salts are selected from the group consisting of citrate and sulfate, citrate and acetate, and sulfate and acetate, respectively, and wherein the concentration of each of the first salt and the second salt in the mixture is between about 0.1 M and about 1.0.
A method of increasing the dynamic capacity of a hydrophobic interaction chromatography column for a protein, comprising mixing a preparation containing the protein with a combination of a first salt and a second salt, and loading the mixture onto a hydrophobic interaction chromatography column, wherein the first and second salts are selected from the group consisting of citrate and sulfate, citrate and acetate and sulfate and acetate, respectively, and wherein the concentration of each of the first and second salts in the mixture is between about 0.1 M and about 1.0 M.
Dependencies are inferred from the claim text.