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US 8,273,566 B2Granted
Methods and apparatuses for conducting assays
AssigneeMeso Scale Technologies, LLC.
InventorsMark A. Billadeau, Jeff D. Debad, Eli N. Glezer, Jonathan K. Leland, Charles A. Wijayawardhana
Life of this patent
Estimating the term…
Abstract
Disclosed are methods for conducting assays of samples, such as whole blood, that may contain cells or other particulate matter. Also disclosed are systems, devices, equipment, kits and reagents for use in such methods. One advantage of certain disclosed methods and systems is the ability to rapidly measure the concentration of an analyte of interest in blood plasma from a whole blood sample without blood separation and hematocrit correction.
Claims36
A method for measuring an analyte of interest comprising acts of:
- (a) exposing a whole blood sample to a binding surface within a flow cell by a back and forth flow of said sample over said binding surface so that at least a portion of the sample contacts the binding surface, wherein said binding surface has at least one binding reagent immobilized thereon and said binding reagent binds said analyte of interest;
- (b) immobilizing an amount of said analyte on said surface;
- (c) measuring said amount of said analyte on said surface;
- and
- (d) determining, from said amount, a measured plasma concentration value of said analyte in said sample that differs from the actual plasma concentration of said analyte in said sample by no more than 20%;
- wherein said determining act does not include a hematocrit correction.
3 more elements
A method for measuring an analyte of interest comprising acts of:
- (a) contacting a whole blood sample with a binding surface within a flow cell by a back and forth flow of said sample over said binding surface, wherein said binding surface has at least one binding reagent immobilized thereon and said binding reagent binds said analyte of interest;
- (b) immobilizing an amount of said analyte on said surface;
- (c) generating an assay signal that is indicative of said amount of said analyte on said surface;
- and
- (d) determining, from said amount, a measured plasma concentration value of said analyte in said sample that differs from the actual plasma concentration of said analyte in said sample by no more than 20%;
- wherein said determining act does not include a hematocrit correction.
3 more elements
A method for performing rapid blood test comprising acts of:
- (a) drawing a sample of blood from a patient to provide a whole blood sample;
- (b) applying said whole blood sample to a cartridge, wherein said cartridge comprises a binding surface within a flow cell by a back and forth flow of said sample over said binding surface so that at least a portion of said sample contacts said binding surface, wherein said binding surface has at least one binding reagent immobilized thereon and said binding reagent binds an analyte of interest;
- (c) flowing said whole blood sample over said surface for a defined interval of time to immobilize an amount of said analyte on said surface;
- (d) measuring said amount of said analyte on said surface;
- (e) determining, from said amount, a measured plasma concentration value of said analyte in said sample that differs from the actual plasma concentration of said analyte in said sample by no more than 20%;
- wherein said determining act does not include a hematocrit correction.
3 more elements
A method for measuring an analyte of interest comprising acts of:
- (a) exposing a whole blood sample comprising particulate matter and a plasma fraction to a binding surface so that at least at least a portion of the sample contacts the binding surface within a flow cell by a back and forth flow of said sample over said binding surface so that at least a portion of said sample contacts said binding surface, wherein said binding surface has at least one binding reagent immobilized thereon and said binding reagent binds an analyte of interest;
- (b) immobilizing an amount of said analyte on said surface;
- (c) measuring said amount of said analyte on said surface;
- and
- (d) determining, from said amount, a measured plasma concentration value of said analyte in said sample that differs from the actual concentration of said analyte in a liquid fraction of said sample by no more than 20%;
- wherein said determining act does not include correction for a volume occupied by particulate matter.
3 more elements
A method for measuring an analyte of interest comprising acts of:
- (a) exposing a whole blood sample comprising particulate matter and a plasma fraction to a binding surface so that at least a portion of the sample contacts the binding surface within a flow cell by a back and forth flow of said sample over said binding surface so that at least a portion of said sample contacts said binding surface, wherein said binding surface has at least one binding reagent immobilized thereon and said binding reagent binds an analyte of interest;
- (b) immobilizing an amount of said analyte on said surface;
- (c) generating an assay signal that is indicative of said amount of said analyte on said surface;
- and
- (d) determining, from said signal, a measured plasma concentration value of said analyte in said sample that differs from the actual plasma concentration of said analyte in said sample by no more than 20%;
- wherein said determining act does not include correction for a volume occupied by particulate matter.
3 more elements
Dependencies are inferred from the claim text.