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AssigneeGevo, Inc.
InventorsCatherine Asleson Dundon, Aristos Aristidou, Andrew Hawkins, Doug Lies, Lynne Albert
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The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
A recombinant yeast microorganism comprising a recombinantly overexpressed polynucleotide encoding a dihydroxy acid dehydratase (DHAD), wherein said recombinant yeast microorganism is engineered to comprise at least one inactivated monothiol glutaredoxin selected from the group consisting of monothiol glutaredoxin-3 (GRX3) and monothiol glutaredoxin-4 (GRX4), and wherein said inactivated monothiol glutaredoxin results from the deletion of one or more nucleotides of an endogenous gene encoding said monothiol glutaredoxin, the insertion of one or more nucleotides into an endogenous gene encoding said monothiol glutaredoxin, or combinations thereof.
Dependencies are inferred from the claim text.