Production of peracids using an enzyme having perhydrolysis activity

A disinfection formulation comprising:

• a) a first mixture comprising
• 1) an enzyme catalyst comprising an enzyme, wherein said enzyme has perhydrolase activity and comprises a signature motif that aligns with a reference sequence SEQ ID NO:
• 2 using CLUSTALW, said signature motif comprising:
• i) an RGQ motif at positions corresponding to positions 118-120 of SEQ ID NO:2;
• ii) a GXSQG motif at positions corresponding to positions 179-183 of SEQ ID NO:2;
• and
• iii) an HE motif at positions corresponding to positions 298-299 of SEQ ID NO:2;
• and
• 2) a substrate selected from the group consisting of methyl lactate, ethyl lactate, methyl glycolate, ethyl glycolate, methyl methoxyacetate, ethyl methoxyacetate, methyl 3-hydroxybutyrate, ethyl 3-hydroxybutyrate, monoacetin, diacetin, triacetin, monopropionin, dipropionin, tripropionin, monobutyrin, dibutyrin, tributyrin, glucose pentaacetate, xylose tetraacetate, acetylated xylan, β-D-ribofuranose-1,2,3,5-tetraacetate, tri-O-acetyl-D-galactal, tri-O-acetyl-glucal, acetylated monosaccharides, acetylated disaccharides, acetylated polysaccharides, and mixtures thereof;
• said first mixture optionally comprising a further component selected from the group consisting of an inorganic or organic buffer, a corrosion inhibitor, a wetting agent, and combinations thereof;
• and
• b) a second mixture comprising a source of peroxygen and water, said second mixture optionally further comprising a chelating agent.