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US 8,273,562 B2Granted
Method for producing 4-hydroxy-L-isoleucine
AssigneeAjinomoto Co., Inc.
InventorsTomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
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Abstract
A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
Claims4
An isolated DNA selected from the group consisting of:
- (a) a DNA comprising the nucleotide sequence of SEQ ID No:
- 1;
- (b) a DNA that hybridizes under stringent conditions with a DNA comprising a nucleotide sequence complementary to the nucleotide sequence of SEQ ID No:
- 1, and wherein said stringent conditions comprise washing at a salt concentration of 0.1×SSC and 0.1% SDS at 37° C., and wherein said DNA encodes a protein having L-isoleucine dioxygenase activity;
- (c) a DNA that encodes a protein comprising the amino acid sequence of SEQ ID No:
- 2;
- (d) a DNA that encodes a protein comprising the amino acid sequence of SEQ ID No:
- 2, but which includes one to 13 substitutions, deletions, insertions, additions, or inversions of one to 13 amino acids and wherein said protein has L-isoleucine dioxygenase activity;
- and
- (e) a DNA that encodes a protein comprising an amino acid sequence that is at least 98% homologous to the amino acid sequence of SEQ ID NO:
- 2, and has L-isoleucine dioxygenase activity.
8 more elements
Dependencies are inferred from the claim text.