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AssigneeCodexis, Inc.
InventorsLorraine Joan Giver, Lisa Marie Newman, Emily Mundorff, Gjalt W. Huisman, Stephane J. Jenne, Jun Zhu, Behnaz Behrouzian, John H. Grate, James Lalonde
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The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.
An engineered or isolated ketoreductase enzyme capable of stereoselectively reducing the 3-oxo group of a 5-hydroxy-3-oxohexanoate to yield the corresponding syn 3,5-dihydroxyhexanoate ester and having increased specific activity as compared to the wild-type ketoreductase enzyme of SEQ ID NO:2, wherein the ketoreductase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
Dependencies are inferred from the claim text.