Human T2R receptors for acetaminophen, ranitidine, strychnine and denatonium and related assays for identifying human bitter taste modulators
AssigneeSenomyx, Inc.
InventorsXiaodong Li, Hong Xu, Hulxian Tang, Qing Li
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Abstract
The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors and which may be used as additives in foods, beverages and medicinals for modifying (blocking) T2R-associated bitter taste.
Claims26
An assay for identifying a compound which modulates a human hT2R75 bitter taste receptor polypeptide that possesses at least 90% sequence identity to the polypeptide contained in SEQ ID NO:
- 15 which comprises:
- i. screening a compound for its effect on the specific binding or activation of said hT2R75 polypeptide by a bitter ligand selected from ranitidine, strychnine and denatonium benzoate, and ii. determining whether said compound modulates hT2R75 associated bitter taste based on its effect on the activation of said receptor by said bitter ligand.
Dependencies are inferred from the claim text.