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US 8,273,533 B2Granted
Patterned spot microarray using photocatalyst and method of manufacturing the same
AssigneeSamsung Electronics Co., Ltd.
InventorsJong-suk Chung, Kyu-youn Hwang, Jeo-young Shim
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Abstract
Provided is a method of preparing a patterned spot microarray using a photocatalyst. The method comprises coating the photocatalyst on a substrate to form a photocatalyst layer, coating a composition comprising a functional group to be connected to a biomolecule on the photocatalyst layer to form an organic layer, spotting the biomolecule on the organic layer, positioning a photomask above a spot of the biomolecule; and irradiating the spot through the photomask to pattern the spot.
Claims12
A method of preparing a patterned spot microarray comprising:
- coating a photocatalyst on a substrate to form a photocatalyst layer;
- coating a composition on the photocatalyst layer to form an organic thin film layer coated on the photocatalyst layer, wherein the organic thin film layer comprises a functional group to be connected to a nucleic acid;
- spotting the nucleic acid on the organic thin film layer and immobilizing the nucleic acid;
- positioning a photomask above a spot of the immobilized nucleic acid;
- and
- irradiating the spot of the immobilized nucleic acid with ultraviolet light through the photomask to form a pattern on the spot of the immobilized nucleic acid,
- wherein nucleic acid irradiated with light through the photomask is oxidized to reduce the size of the nucleic acid spot, and
- wherein target nucleic acid present in a sample does not hybridize to the patterned spot microarray in an area where the nucleic acid has been irradiated with light through the photomask.
5 more elements
Dependencies are inferred from the claim text.