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US 8,273,526 B2Granted
Method of isolating stem and progenitor cells from placenta
AssigneeChildren's Hospital & Research Center at Oakland
InventorsFrans A. Kuypers, Vladimir B. Serikov
Life of this patent
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Abstract
The present invention provides methods of cryopreserving stem and progenitor cells in a mammalian placenta; and methods of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta. Cells obtained by carrying out the methods can be used in a variety of therapeutic applications.
Claims18
A method of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta, the method comprising:
- a) perfusing a cryopreserved mammalian placenta with a collection solution comprising an effective amount of an agent that induces mobilization of a stem and/or progenitor cell into a blood vessel of the placenta, wherein the mobilization agent is AMD-3100, a biocompatible non-ionic surfactant, an antibody that binds specifically to an adhesion molecule and that inhibits cell adhesion, a thrombolytic enzyme, or ethylenediamine tetraacetic acid;
- and
- b) collecting the mobilized cells from the blood vessel, wherein the collected cells comprise fetal stem and progenitor cells.
Dependencies are inferred from the claim text.