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US 8,273,372 B2Granted
Extracellular matrix composition
AssigneeRestoration of Appearance & Function Trust
InventorJulian Dye
Life of this patent
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Abstract
The invention relates to a process for preparing an extracellular matrix composition comprising cross-linked fibrinogen or a derivative thereof, to an extracellular matrix composition obtained by the process and to the use of the composition in wound healing, tissue regeneration or as a tissue engineering scaffold.
Claims21
A process for preparing an extracellular matrix composition which comprises:
- (a) mixing an aqueous solution of fibrinogen with a coagulating agent and a bulking agent and a foaming agent, wherein the mixing step (a) is achieved by foaming;
- (b) incubating the mixture obtained in step (a) with a cross-linking agent selected from:
- carbodiimide coupling agents including N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC);
- N-hydroxysuccinimide (NHS), azide coupling agents;
- diisocyanate cross-linking agents including hexamethylene diisocyanate;
- epoxide cross-linking agents including epi-chlorhydrin, glycidylethers and glycidylamines;
- and aldehyde cross-linking agents including formaldehyde, glutaraldehyde and glyoxal;
- and
- (c) washing the cross-linked composition obtained in step (b) to remove the cross-linking agent.
6 more elements
Dependencies are inferred from the claim text.