Antigen-carbohydrate conjugates
AssigneesMassachusetts Institute of Technology, Whitehead Institute for Biomedical Research, The General Hospital Corporation
InventorsNir Hacohen, Eddie Adams, Peter Seeberger
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Abstract
The present invention generally relates to compositions comprising antigen-carbohydrate conjugates and methods of immune modulation featuring these reagents.
Claims49
A composition comprising at least one mannose oligosaccharide conjugated to a molecule selected from the group consisting of a protein or glycoprotein, a lipid, chemically modified lipid, or glycolipid, polysaccharide, or small molecule, wherein the composition is capable of binding to a protein present on a dendritic cell.
A composition comprising at least one carbohydrate conjugated to an antigen, wherein the composition is capable of binding to a carbohydrate binding protein present on an antigen presenting cell, and the carbohydrate is a mannose oligosaccharide.
A composition comprising an antigen-carbohydrate conjugate that binds a lectin expressed on a human dendritic cell in vivo or ex vivo, the conjugate comprising mannose in a pharmaceutically acceptable carrier.
An immunogenic composition comprising at least one mannose oligosaccharide conjugated to an antigen in a pharmaceutically acceptable excipient, wherein the mannose oligosaccharide-antigen conjugate binds a lectin that is expressed on a dendritic cell wherein the composition is capable of modulating an immune response.
A method of immunization, comprising:
- administering to a subject a composition comprising a conjugate of an antigen and a mannose oliqosaccharide that binds a lectin expressed on an antigen-presenting cell, wherein the composition is in an amount effective to immunize the subject to the antigen.
A method of enhancing antigen presentation by a cell, the method comprising contacting an antigen-presenting cell with at least one mannose oligosaccharide conjugated to an antigen or antigen mimetic, wherein the contacting enhances antigen presentation by the antigen-presenting cell.
A method of increasing T cell proliferation in a subject, the method comprising contacting an antigen-presenting cell with at least one mannose oligosaccharide conjugated to an antigen or antigen mimetic, wherein the contacting enhances antigen presentation by the antigen-presenting cell.
A method of generating an antigen mannose oligosaccharide conjugate, the method comprising:
- conjugating an antigen to a mannose oligosaccharide able to bind a lectin that is expressed on antigen-presenting cells but is not substantially expressed on non-antigen-presenting cells.
A method for producing a conjugated antigen, the method comprising:
- providing a lectin that is expressed on antigen-presenting cells but is not substantially expressed on non-antigen-presenting cells;
- screening a carbohydrate library to identify carbohydrates able to bind the lectin;
- and conjugating an antigen to one of the identified carbohydrates able to bind the lectin.
A method of immunization, comprising:
- administering, to a human or mammalian subject, a composition comprising a conjugate of an antigen and a carbohydrate, comprising mannose, that is able to bind a lectin that is expressed on antigen-presenting cells, in an amount effective to immunize the subject to the antigen.
A method for conjugating an antigen to a carbohydrate, the method comprising:
- providing a lectin that is expressed on antigen-presenting cells but is not substantially expressed on non-antigen-presenting cells;
- screening a carbohydrate library to identify carbohydrates able to bind the lectin;
- and conjugating an antigen to one of the identified carbohydrates able to bind the lectin.
Dependencies are inferred from the claim text.