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US 8,273,345 B2Granted
Targeted gene delivery to dendritic cells
AssigneeCalifornia Institute of Technology
InventorsPin Wang, Lili Yang, David Baltimore
Life of this patent
Estimating the term…
Abstract
Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.
Claims8
A method of delivering a lentivirus encoding a tumor antigen to elicit an antigen-specific cytotoxic T-cell response in a mammal comprising:
- subcutaneously or intradermally administering to said mammal a replication deficient lentivirus pseudotyped with a virus envelope comprising a modified E2 alphavirus glycoprotein comprising a modification that reduces binding of said E2 to heparan sulfate, wherein the lentivirus comprises a polynucleotide encoding a tumor antigen, and wherein the lentivirus comprising the modified E2 alphavirus glycoprotein more efficiently transduces dendritic cells expressing DC-SIGN relative to cell types not expressing DC-SIGN, in an amount sufficient to elicit an antigen-specific cytotoxic T-cell response to the tumor antigen.
Dependencies are inferred from the claim text.