Cyanine compounds, compositions including these compounds and their use in cell analysis
AssigneeShenzhen Mindray Bio-Medical Electronics Co., Ltd.
InventorsYumei Zhao, Jianhui Shao, Yang Zhao, Ting Lei, Bing Xu
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Abstract
A compound having the general formula I or a conjugate thereof, wherein various groups are as defined in the specification. A composition includes: (i) a compound having the general formula I or a conjugate thereof; and (ii) at least one surfactant selected from cationic surfactants and nonionic surfactants. Also disclosed is a preparation method for the composition and a kit comprising the composition. Further disclosed is a method for identifying and differentiating erythroblasts, basophils and lymphocytes simultaneously using the composition according to the present disclosure.
Claims27
A compound having the general formula I:
- wherein
- Ar1 is a benzene ring;
- X is selected from at least one of the following:
- —O—, —S—, and
- R1 and R3 are independently selected from C3-20 alkenyl or C3-20 alkynyl;
- R2 is selected from at least one of the following:
- hydrogen, carboxy, sulphonyl, C1-20 saturated or unsaturated alkyl or C1-20 saturated or unsaturated alkoxyl;
- and
- Y− is an anion.
A composition, said composition comprising:
- (i) a compound according to formula I
- wherein
- Ar1 is an aromatic ring;
- X is selected from at least one of the following:
- —O—, —S—, and
- R1 and R3 are independently selected from C3-20 alkenyl or C3-20 alkynyl;
- R2 is selected from at least one of the following:
- hydrogen, carboxy, sulphonyl, C1-20 saturated or unsaturated alkyl or C1-20 saturated or unsaturated alkoxyl;
- Y− is an anion,
- or a conjugate thereof, and
- (ii) at least one surfactant selected from cationic surfactants or nonionic surfactants, wherein said cationic surfactants are quaternary ammonium salt-type cationic surfactants having the following general formula II:
- wherein
- R8 is alkyl or alkenyl having 6 to 14 carbon atoms;
- R9 and R10 are independently selected from C1-4 alkyl or C2-4 alkenyl;
- R11 is selected from C1-4 alkyl, C2-4 alkenyl or benzyl;
- and
- B is a halide ion, and
- wherein said nonionic surfactants are polyoxyethylene-type nonionic surfactants having the general formula III:
- RA-RB—(CH2CH2O)n—H Formula III
- wherein
- RA is alkyl or alkenyl having 8 to 23 carbon atoms;
- RB is selected from —O—,
- or —COO—;
- and
- n is an integer of 8-30.
A method for identifying erythroblasts and differentiating and counting leukocytes simultaneously, wherein said method comprises the following steps:
- (a) mixing a blood sample with a composition comprising a compound according to Formula I
- wherein
- Ar1 is an aromatic ring;
- X is selected from at least one of the following:
- —O—, —S—, and
- R1 and R3 are independently selected from C3-20 alkenyl or C3-20 alkynyl;
- R2 is selected from at least one of the following:
- hydrogen, carboxy, sulphonyl, C1-20 saturated or unsaturated alkyl or C1-20 saturated or unsaturated alkoxyl;
- and
- Y− is an anion, to form a cell suspension, wherein the compound of Formula I enters cells and combines with nucleic acids;
- (b) detecting scattered light signals and fluorescence signals from cells in the cell suspension;
- and
- (c) differentiating erythroblasts and leucocytes in terms of difference of scattered light signals and fluorescence signals.
A compound selected from:
Dependencies are inferred from the claim text.