# US 8,273,227 B2 · Sensor for in vitro determination of glucose

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- Number: US 8,273,227 B2
- Document ID: US8273227B2
- Status: Granted
- Kind: B2
- Application number: 11/929,906
- Filed: 2007-10-30
- Published: 2008-11-20
- Granted: 2012-09-25
- Earliest claimed: 1998-03-04
- Assignees: Abbott Diabetes Care Inc.
- Inventors: James Say; Michael F. Tomasco; Adam Heller; Yoram Gal; Behrad Aria; Ephraim Heller; Phillip John Plante; Mark S. Vreeke
- Web page: https://patentgrep.com/patent/US8273227B2

## CPC

- A61B 5/14735: {comprising an immobilised reagent}
- A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement}
- A61B 5/14865: {invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors}
- C12Q 1/001: {Enzyme electrodes}
- G01N 27/3272: {Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52)}
- G01N 33/5438: {Electrodes}
- G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding}
- G01N 33/66: involving blood sugars, e.g. galactose
- A61B 2562/0271: Thermal or temperature sensors
- A61B 2562/125: characterised by the manufacture of electrodes
- Y10T 29/42: Piezoelectric device making
- Y10T 29/49002: Electrical device making
- Y10T 29/49004: including measuring or testing of device or component part
- Y10T 29/49007: Indicating transducer
- Y10T 29/49117: Conductor or circuit manufacturing
- Y10T 29/49128: Assembling formed circuit to base
- Y10T 29/49155: Manufacturing circuit on or in base
- Y10T 29/49208: by assembling plural parts
- Y10T 29/49798: Dividing sequentially from leading end, e.g., by cutting or breaking
- Y10T 156/1064: Partial cutting [e.g., grooving or incising]
- Y10T 156/1082: Partial cutting bonded sandwich [e.g., grooving or incising]
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A process for the manufacture of small sensors with reproducible surfaces, including electrochemical sensors. One process includes forming channels in the surface of a substrate and disposing a conductive material in the channels to form an electrode. The conductive material can also be formed on the substrate by other impact and non-impact methods. In a preferred embodiment, the method includes cutting the substrate to form a sensor having a connector portion and a transcutaneous portion, the two portions having edges that define one continuous straight line.

## Claims

### Claim 1

A sensor for the in vitro determination of glucose level in a sample, the sensor comprising:
a substrate comprising a first recessed channel;
a first conductive material to provide an electrode disposed in the recessed channel on the substrate to substantially fill the channel such that the substrate and the conductive material form a substantially flat surface,
wherein a catalyst is mixed or dispersed in said first conductive material,
wherein the catalyst includes an enzyme, wherein the enzyme is mixed in a carbon ink to form a mixture,
wherein the sensor generates an electrical signal associated with a level of glucose in a volume sample of about 1 microliter or less.

### Claim 2 (depends on 1)

The sensor of claim 1, wherein the catalyst forms a conductive trace associated with a working electrode.

### Claim 3 (depends on 1)

The sensor of claim 1, wherein the substrate comprises a second recessed channel and a second conductive material is applied in the second recessed channel.

### Claim 4 (depends on 3)

The sensor of claim 3, wherein the first conductive material and the second conductive material are the same.

### Claim 5 (depends on 3)

The sensor of claim 3, wherein the first conductive material and the second conductive material are different.

### Claim 6 (depends on 1)

The sensor of claim 1, wherein the substrate includes an indentation.

### Claim 7 (depends on 1)

The sensor of claim 1, wherein a trace is associated with the electrode, the trace having a width of about 25 microns to 250 microns.

### Claim 8 (depends on 7)

The sensor of claim 7, wherein the trace has a width of about 75 microns or less.

### Claim 9 (depends on 8)

The sensor of claim 8, wherein the trace has a width of about 50 microns or less.

### Claim 10 (depends on 9)

The sensor of claim 9, wherein the trace has a width of about 25 microns or less.

### Claim 11 (depends on 1)

The sensor of claim 1, wherein the sensor is coupled to a skin piercer.

### Claim 12 (depends on 1)

The sensor of claim 1, comprising a tapered end.

### Claim 13 (depends on 1)

The sensor of claim 1, wherein the enzyme is glucose oxidase.

### Claim 14 (depends on 1)

The sensor of claim 1, wherein the enzyme is glucose dehydrogenase.

### Claim 15 (depends on 1)

The sensor of claim 1, wherein the enzyme is pyrroloquinoline quinone glucose dehydrogenase.