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US 8,268,303 B2Granted
Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
AssigneeAdvanced Cell Technology, Inc.
InventorsIrina V. Klimanskaya, Robert P. Lanza
Life of this patent
Estimating the term…
Abstract
This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
Claims42
A method for the differentiation of hES cells into RPE cells, said method comprising:
- a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells;
- b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- and
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, express RPE65.
1 more element
A method of administering human RPE cells to an individual in need thereof, comprising:
- a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells;
- b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, and express RPE65;
- and
- d) introducing the human RPE cells into the individual in need thereof.
2 more elements
A method for the treatment of Parkinson's disease in an individual in need thereof, comprising:
- a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells;
- b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, and express RPE65;
- and
- d) introducing the human RPE cells into the individual in need thereof.
2 more elements
A method for the differentiation of hES cells into RPE cells, said method comprising:
- a) allowing hES cell cultures to overgrow and form a thick multilayer of cells;
- b) culturing the thick multilayer of cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- and
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, express RPE65.
1 more element
A method of administering human RPE cells to an individual in need thereof, comprising:
- a) allowing hES cell cultures to overgrow and form a thick multilayer of cells;
- b) culturing the thick multilayer of cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, and express RPE65;
- and
- d) introducing the human RPE cells into the individual in need thereof.
2 more elements
A method for the treatment of Parkinson's disease in an individual in need thereof, comprising:
- a) allowing hES cell cultures to overgrow and form a thick multilayer of cells;
- b) culturing the thick multilayer of cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, and express RPE65;
- and
- d) introducing the human RPE cells into the individual in need thereof.
2 more elements
A method for the differentiation of hES cells into RPE cells, said method comprising:
- a) forming an embryoid body (EB) from hES cells;
- b) culturing the EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- and
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, express RPE65.
1 more element
A method of administering human RPE cells to an individual in need thereof, comprising:
- a) forming an embryoid body (EB) from hES cells;
- b) culturing the EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, and express RPE65;
- and
- d) introducing the human RPE cells into the individual in need thereof.
2 more elements
A method for the treatment of Parkinson's disease in an individual in need thereof, comprising:
- a) forming an embryoid body (EB) from hES cells;
- b) culturing the EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−, bestrophin+, CRALBP+, PEDF+, and express RPE65;
- and
- d) introducing the human RPE cells into the individual in need thereof.
2 more elements
Dependencies are inferred from the claim text.