Systems and methods for baseline correction using non-linear normalization

A system for generating a normalized amplification profile, comprising:

• a processor;
• and
• a memory for storing instructions executable by the processor, the instructions including instructions for:
• receiving emission data detected in an amplification reaction,
• identifying a baseline signal in the emission data,
• generating a normalization quantity based on the emission data and the identified baseline signal,
• generating the normalized amplification profile based on a ratio of the emission data to the normalization quantity,
• generating a threshold cycle based on the normalized amplification profile, and
• quantifying an original sample amount based on the threshold cycle.

A non-transitory computer-readable storage medium encoded with instructions, executable by a processor, for generating a normalized amplification profile, the instructions comprising instructions for:

• receiving emission data detected in an amplification reaction,
• identifying a baseline signal in the emission data,
• generating a normalization quantity based on the emission data and the identified baseline signal,
• generating the normalized amplification profile based on a ratio of the emission data to the normalization quantity,
• generating a threshold cycle based on the normalized amplification profile, and
• quantifying an original sample amount based on the threshold cycle.