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US 8,265,878 B2Granted
Method for rapid detection and identification of bioagents
AssigneeIbis Bioscience, Inc.
InventorsDavid J. Ecker, Richard H. Griffey, Rangarajan Sampath, Steven A. Hofstadler, John McNeil
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Abstract
Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.
Claims24
A system, comprising:
- a) a nucleic acid amplification component;
- b) a base composition determination component comprising a processor wherein said base composition identifies the number of, but not the nucleic acid gene sequence order of, A residues, C residues, T residues, G residues, U residues, analogs thereof and/or mass tag residues thereof;
- and
- c) a base composition identification component comprising a processor and a database of base compositions from a plurality of bioagents wherein said base compositions identify the number of, but not the nucleic acid gene sequence order of, A residues, C residues, T residues, G residues, U residues, analogs thereof and/or mass tag residues thereof;
- said database comprising a plurality of calculated or measured base compositions of amplification products of bioagents obtained by amplification of at least one target sequence of a bioagent nucleic acid gene sequence using a primer pair that hybridizes to the at least one target sequence region of a plurality of bioagents said target sequence region comprising two conserved regions that are hybridizable with the primer pair and that flank a variable region that uniquely varies between a plurality of bioagents.
2 more elements
Dependencies are inferred from the claim text.