Method for evaluating cell detachment, method of detaching cultured cell, apparatus for evaluating cell detachment and automated cell culture apparatus
AssigneeKawasaki Jukogyo Kabushiki Kaisha
InventorsOsamu Ohji, Toshihisa Doi
Life of this patent
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Abstract
A novel method for evaluating cell detachment which can be used in passage operation and the like in culture of adhesive cells; a method of detaching a cultured cell utilizing the same; an apparatus for evaluating cell detachment for carrying out the aforementioned method for evaluating cell detachment; and an automated cell culture apparatus in which the apparatus is used are provided.
Claims14
A method for evaluating cell detachment in which cell detachment from a culture vessel is evaluated, the method comprising the steps of:
- taking a first image by imaging a certain position in a culture vessel;
- taking a second image by imaging the certain position after taking the first image and also after allowing the culture liquid in the culture vessel to flow;
- and
- evaluating, via an overall control apparatus, the cell detachment from the culture vessel by determining a cell area ratio A for a section where the cell is present in the first image, a cell area ratio B for a section where the cell is present in the second image, and a common area ratio C for a section where the cell is present in both the first image and the second image, and judging the cell detachment based on the cell area ratio A, the cell area ratio B and the common area ratio C.
A method of detaching culture cells, comprising the steps of:
- placing a detachment solution into a culture vessel for allowing the cultured cell to be detached from the culture vessel;
- taking a first image by imaging a certain position in a culture vessel;
- taking a second image by imaging the certain position after taking the first image and also after allowing the culture liquid in the culture vessel to flow;
- evaluating, via an overall control apparatus, the cell detachment from the culture vessel by determining a cell area ratio A for a section where the cell is present in the first image, a cell area ratio B for a section where the cell is present in the second image, and a common area ratio C for a section where the cell is present in both the first image and the second image, and judging the cell detachment based on the cell area ratio A, the cell area ratio B and the common area ratio C;
- and
- placing a detachment stop solution into the culture vessel.
An apparatus for evaluating cell detachment comprising:
- a vibration exciter which allows the culture liquid in the culture vessel to turn into its flowing state;
- a device for taking an image for obtaining a first image by imaging a certain position in a culture vessel and a second image by imaging the certain position after taking the first image and also after allowing the culture liquid in the culture vessel to flow;
- and
- a cell detachment evaluation means for evaluating the cell detachment from the culture vessel by determining a cell area ratio A for a section where the cell is present in the first image, a cell area ratio B for a section where the cell is present in the second image, and a common area ratio C for a section where the cell is present in both the first image and the second image, and judging the cell detachment based on the cell area ratio A, the cell area ratio B and the common area ratio C.
Dependencies are inferred from the claim text.