Determination of a change in a cell population
AssigneeUnisense FertiliTech A/S
InventorsNiels B. Ramsing, Jorgen Berntsen
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Abstract
The present invention relates to a method and a system for determination of a change in a cell population, as well as a method for using said method and system for estimating a quality measure of embryos and for selecting embryos for in vitro fertilization, said method comprising the steps of sequentially acquiring at least two images of the cell population, comparing at least a part of the at least two images obtaining at least one difference image, computing a parameter from the at least one difference image, based on said computed parameter determining whether a change has occurred.
Claims49
A method for determining a magnitude of a change in a cell population comprising at least one cell, said method comprising the steps of
- a) sequentially acquiring at least two images of the cell population
- b) comparing at least a part of the at least two images, thereby obtaining at least one difference image
- c) computing a parameter from the at least one difference image, wherein said parameter provides information of the magnitude of a change, and
- d) based on said computed parameter determining the magnitude of the change,
- wherein the parameter computed from the difference image is selected from the group of Sum of absolute values for pixels in the difference image;
- Mean absolute value for pixels in the difference image;
- Median absolute value for pixels in the difference image;
- Sum of squared values;
- Mean squared value for pixels in the difference image;
- Median of squared value for pixels in the difference image;
- Variance of values for pixels in the difference image;
- Standard deviation of values for pixels in the difference image;
- Values for different percentiles in the histogram of the image;
- Difference Image minimum and maximum values;
- and Range or variance of histogram or another parameter derived from a combination of one or more of these parameters.
A method for determining a the quality of a cell population comprising at least one cell, said method comprising the steps of
- e) sequentially acquiring at least two images of the cell population:
- f) comparing at least a part of the at least two images, thereby obtaining at least one difference image
- g) computing a parameter from the at least one difference image;
- wherein said parameter provides information of the magnitude of a change, and
- h) based on said computed parameter determining file quality of the cell population,
- wherein the determining embryo quality comprising monitoring the embryo for a time period, said time period having a length sufficient to comprise at least one cell division period and at least a part of an inter-division period, and determining the length of the at least one cell division period and/or determining the cellular movement during the inter-division period and/or determining length of time period of cellular movement during an inter-division period thereby obtaining an embryo quality measure.
Dependencies are inferred from the claim text.