Back to results
US 8,263,759 B2Granted
Sets of probes and primers for the diagnosis of select cancers
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsJaved Khan, Markus Ringner, Carsten Peterson, Paul Meltzer
Life of this patent
Estimating the term…
Abstract
A method of diagnosing a disease that includes obtaining experimental data on gene selections. The gene selection functions to characterize a cancer when the expression of that gene selection is compared to the identical selection from a noncancerous cell or a different type of cancer cell. The invention also includes a method of targeting at least one product of a gene that includes administration of a therapeutic agent. The invention also includes the use of a gene selection for diagnosing a cancer.
Claims12
An article of manufacture for detecting the expression of a set of human genes that comprise SFRP1, GATA2, TNNT2, FGFR4, PTPN13, TNFAIP6, CAV1, and PRKAR2b, consisting essentially of:
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a SFRP1 nucleic acid at a temperature of at least 55° C., wherein SFRP1 nucleic acid has the nucleotide sequence of SEQ ID NO:42,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a GATA 2 nucleic acid at a temperature of at least 55° C., wherein GATA 2 nucleic acid has the nucleotide sequence of SEQ ID NO:44,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a TNNT2 nucleic acid at a temperature of at least 55° C., wherein TNNT2 nucleic acid has the nucleotide sequence of SEQ ID NO:74,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a FGFR4 nucleic acid at a temperature of at least 55° C., wherein FGFR4 nucleic acid has the nucleotide sequence of SEQ ID NO:71,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a PTPN13 nucleic acid at a temperature of at least 55° C., wherein PTPN13 nucleic acid has the nucleotide sequence of SEQ ID NO:16,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a TNFAIP6 nucleic acid at a temperature of at least 55° C., wherein TNFAIP6 nucleic acid has the nucleotide sequence of SEQ ID NO:18,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a CAV1 nucleic acid at a temperature of at least 55° C., wherein CAV1 nucleic acid has the nucleotide sequence of SEQ ID NO:19, and
- a probe or a primer, or both, or a set of primers or probes or both, that specifically hybridize to a PRKAR2B nucleic acid at a temperature of at least 55° C., wherein PRKAR2B nucleic acid has the nucleotide sequence of SEQ ID NO:95, and
- one or more primers or probes or both that specifically hybridize at a temperature of at least 55° C. to one or more different nucleic acid sequences selected from the group consisting of SEQ ID NO:1-2, 4-8, 10-15, 17, 20-33, 35-41, 43, 45, 47, 49-70, 72-73, 75-94, 96, and 100-208.
6 more elements
An article of manufacture comprising:
- reagents for detecting the expression of a set of human genes that comprise SFRP1, GATA2, TNNT2, FGFR4, PTPN13, TNFAIP6, CAV1, and PRKAR2b, wherein the reagents comprise:
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a SFRP1 nucleic acid at a temperature of at least 55° C., wherein SFRP1 nucleic acid has the nucleotide sequence of SEQ ID NO:42,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a GATA 2 nucleic acid at a temperature of at least 55° C., wherein GATA 2 nucleic acid has the nucleotide sequence of SEQ ID NO:44,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a TNNT2 nucleic acid at a temperature of at least 55° C., wherein TNNT2 nucleic acid has the nucleotide sequence of SEQ ID NO:74,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a FGFR4 nucleic acid at a temperature of at least 55° C., wherein FGFR4 nucleic acid has the nucleotide sequence of SEQ ID NO:71,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a PTPN13 nucleic acid at a temperature of at least 55° C., wherein PTPN13 nucleic acid has the nucleotide sequence of SEQ ID NO:16,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a TNFAIP6 nucleic acid at a temperature of at least 55° C. , wherein TNFAIP6 nucleic acid has the nucleotide sequence of SEQ ID NO:18,
- a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a CAV1 nucleic acid at a temperature of at least 55° C., wherein CAV1 nucleic acid has the nucleotide sequence of SEQ ID NO:19, and
- a probe or a primer, or both, or a set of primers or probes or both, that specifically hybridize to a PRKAR2B nucleic acid at a temperature of at least 55° C., wherein PRKAR2B nucleic acid has the nucleotide sequence of SEQ ID NO:95, and
- one or more primers or probes or both that specifically hybridize at a temperature of at least 55° C. to one or more different nucleic acid sequences selected from the group consisting of SEQ ID NO:1-2, 4-8, 10-15, 17, 20-33, 35-41, 43, 45, 47, 49-70, 72-73, 75-94, 96, and 100-208, wherein the article of manufacture does not include any additional primers or probes other than the above primers or probes.
7 more elements
Dependencies are inferred from the claim text.