Leaf-specific chlorophyll a/b binding protein gene promoter from oil palm
AssigneeMalaysian Palm Oil Board
InventorsPek Lan Chan, Siti Nor Akmar Abdullah
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Abstract
The present invention discloses a promoter sequence of light-harvesting chlorophyll a/b binding protein pGWLS01 isolated from the oil palm leaf. This promoter enables the manipulation of oil palm leaves for the production of high value-added products via genetic engineering tools. The novel features of the promoter itself which regulate high and specific expression of foreign genes in the leaves will avoid the interference of novel products in the commodity oil extracted from mesocarp and kernel tissues. Furthermore, the promoter is also potentially useful in the production of insect-resistant palm.
Claims15
A nucleic acid construct comprising an isolated nucleic acid comprising the sequence of SEQ ID NO:
- 1 operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues, wherein the recombinant nucleic acid encodes a protein that imparts insect resistance, production of bioplastic, production of nutraceutical products, production of pharmaceutical macromolecules including therapeutic and diagnostic proteins, antibodies and vaccines, or results in an increase in photosynthetic rate of a plant.
A vector comprising a nucleic acid construct comprising an isolated nucleic acid comprising the sequence of SEQ ID NO:
- 1 operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues.
A cell comprising a nucleic acid construct comprising an isolated nucleic acid comprising the sequence of SEQ ID NO:
- 1 operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues.
A transgenic plant comprising a nucleic acid construct comprising an isolated nucleic acid comprising the sequence of SEQ ID NO:
- 1 operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues.
A nucleic acid construct comprising a fragment of the nucleic acid of SEQ ID NO:
- 1 with promoter activity, wherein the fragment comprises the following elements depicted in FIG. 12:
- GATA I box, GATA II box, CCAAT box, and the G-box, operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues, wherein the recombinant nucleic acid encodes a protein that imparts insect resistance, production of bioplastic, production of nutraceutical products, production of pharmaceutical macromolecules including therapeutic and diagnostic proteins, antibodies and vaccines, or results in an increase in photosynthetic rate of a plant.
A vector comprising a nucleic acid construct comprising a fragment of the nucleic acid of SEQ ID NO:
- 1 with promoter activity, wherein the fragment comprises the following elements depicted in FIG. 12:
- GATA I box, GATA II box, CCAAT box, and the G-box, operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues.
A cell comprising a nucleic acid construct comprising a fragment of the nucleic acid of SEQ ID NO:
- 1 with promoter activity, wherein the fragment comprises the following elements depicted in FIG. 12:
- GATA I box, GATA II box, CCAAT box, and the G-box, operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues.
A transgenic plant comprising a nucleic acid construct comprising a fragment of the nucleic acid of SEQ ID NO:
- 1 with promoter activity, wherein the fragment comprises the following elements depicted in FIG. 12:
- GATA I box, GATA II box, CCAAT box, and the G-box, operably linked to a recombinant nucleic acid, wherein the nucleic acid construct when introduced into leaf tissues and/or photosynthetic tissues, stimulates expression of the recombinant nucleic acid in the leaf tissues and/or photosynthetic tissues.
Dependencies are inferred from the claim text.