US 8,263,756 B2 · Method of gene transfer via vascular system or ureter

Abstract

The present invention provides a method for gene transfer through the vascular system or a ureter. In a preferable embodiment of this invention, an aqueous solution containing an expression vector comprising a desired gene is injected through the vascular system or a ureter of a desired organ to introduce the gene into the injected area. Specifically, the method of the present invention is effective for ex vivo gene therapy wherein the hepatocyte growth factor (HGF) gene is introduced by electroporation into a kidney to be transplanted. According to the present invention, the HGF functions continuously on the kidney to be transplanted, and the HGF within the treated area does not affect other organs of the body. Thus, the method of the present invention is a safe and effective treatment for maintaining the survival of transplanted kidneys.

Claims42

Dependent claims

A method for introducing a nucleic acid into a kidney, said method comprising the steps of

(a) injecting an aqueous solution containing a nucleic acid into a renal artery, a renal vein, or a ureter of a kidney;
(b) setting the whole kidney between forceps-type electrodes;
and
(c) applying electric stimulation at a voltage within the range of 20V to 75V to the whole kidney, thereby introducing the nucleic acid into the kidney.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

10/474,142Filed 22 Mar 2002

Priority

JP 2001-1090516 Apr 2001

Published

US 2004/0175370 A19 Sep 2004

Granted

11 Sep 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C07K 14/4753
Growth factors; Growth regulators: hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
A61K 38/1833
Growth factors; Growth regulators: hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
A61K 48/00
Medicinal preparations containing genetic material which is inserted into cells of the
A61K 48/0075
Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy:…

Method of gene transfer via vascular system or ureter

Life of this patent

Method of gene transfer via vascular system or ureter

Life of this patent

Abstract

Claims42