Method for removing a protein from a metal chelate resin
AssigneeDaedalus Innovations LLC
InventorRonald W. Peterson
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Abstract
The methods disclosed herein are useful for achieving higher protein concentrations in reverse micelle solutions by extracting a protein from a metal chelate resin using a reverse micelle solution comprising a polyamine that competitively binds to the metal chelate resin, allowing the protein to elute from the resin. The extracted protein in the reverse micelle solution can then be effectively analyzed, for example using NMR spectroscopy.
Claims20
A method for extracting a protein from a metal chelate resin, comprising mixing a protein loaded metal chelate resin with a reverse micelle solution comprising polyethyleneimine (PEI) to thereby extract the protein from the metal chelate resin into the reverse micelle solution.
A method for introducing a protein into a reverse micelle solution, comprising a) loading a protein on a metal chelate resin in an aqueous phase;
- b) reducing the hydration of the protein loaded metal chelate resin;
- c) mixing the resultant protein loaded metal chelate resin with a reverse micelle solution comprising polyethyleneimine (PEI) to thereby extract the protein from the metal chelate resin into the reverse micelle solution.
Dependencies are inferred from the claim text.