Methods for detection of methyl-CpG dinucleotides
AssigneeBrookhaven Science Associates, LLC
InventorJohn J. Dunn
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Abstract
The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5′-CMeCpGG-3′. Methods for making and using the rMcrA protein, and derivatives thereof are provided.
Claims7
An agent which specifically binds 5′-CMeCpGG-3′ DNA sequences, in vitro, comprising recombinant full-length, active McrA protein (rMcrA) or fusion derivative thereof, which protein or derivative thereof is more than about 90% pure.
An agent which specifically binds 5′-CMeCpGG-3′ DNA sequences, in vitro, comprising recombinant full-length, active McrA protein (rMcrA) which protein is more than about 90% pure.
Dependencies are inferred from the claim text.