Gene silencing of the brother of the regulator of imprinted sites (BORIS)
AssigneeVendevia Group, LLC
InventorsBoris N. Reznik, Christopher J. Dougherty, Thomas Ichim
Life of this patent
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Abstract
The present invention provides methods and compositions useful for inhibiting expression of the gene encoding the transcription factor, Brother of the Regulatory of Imprinted Sites (BORIS) by RNA interference. Methods of the present invention can be used to silence BORIS in cancer cells, which results in apoptosis and may be useful as for treating cancer in mammals. The methods of the invention directed to cancer therapy can be used alone or in combination with standard cancer treatments such as surgery, radiation, chemotherapy, and immunotherapy.
Claims19
A method of inhibiting expression of Brother of the Regulator of Imprinted Sites (BORIS) in a cell comprising introducing into the cell a small interfering RNA (siRNA) molecule that directs cleavage of BORIS mRNA, thereby inhibiting expression of BORIS in the cell, wherein the siRNA molecule is a double-strand RNA, RNA-DNA chimera or RNA-DNA hybrid, and wherein one strand of the siRNA has the nucleotide sequence of SEQ ID NOs:59.
A method of inhibiting expression of Brother of the Regulator of Imprinted Sites (BORIS) in a cell comprising introducing into the cell a small interfering RNA (siRNA) molecule that directs cleavage of BORIS mRNA, thereby inhibiting expression of BORIS in the cell, wherein the siRNA molecule is a double-strand RNA, RNA-DNA chimera or RNA-DNA hybrid, and wherein one strand of the siRNA has the nucleotide sequence of SEQ ID NOs:60.
An siRNA molecule that directs cleavage of a BORIS mRNA comprising the polynucleotide sequence of SEQ ID NO:60.
A method of inhibiting expression of a BORIS variant in a cell comprising
- a) detecting expression of a BORIS variant in the cell;
- and
- b) introducing into the cell a small interfering siRNA molecule that directs cleavage of an mRNA encoding the variant BORIS, thereby inhibiting expression of the variant BORIS in the cell, wherein the siRNA molecule is a double-strand RNA, RNA-DNA chimera or RNA-DNA hybrid, and wherein one strand of the siRNA has the nucleotide sequence of SEQ ID NO:59.
A method of inhibiting expression of a BORIS variant in a cell comprising
- a) detecting expression of a BORIS variant in the cell;
- and
- b) introducing into the cell a small interfering siRNA molecule that directs cleavage of an mRNA encoding the variant BORIS, thereby inhibiting expression of the variant BORIS in the cell, wherein the siRNA molecule is a double-strand RNA, RNA-DNA chimera or RNA-DNA hybrid, and wherein one strand of the siRNA has the nucleotide sequence of SEQ ID NO:60.
Dependencies are inferred from the claim text.