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US 8,263,569 B2Granted
Gene silencing
AssigneePlant Biosciences Limited
InventorsDavid Charles Baulcombe, Andrew John Hamilton
Life of this patent
Estimating the term…
Abstract
Methods are disclosed for gene silencing (e.g. post transcriptional gene silencing) in an organism using small RNA molecules.
Claims17
A method of silencing a gene in cells which method comprises introducing into said cells a composition that consists essentially of isolated short antisense RNA molecules (SARMs) and isolated short sense RNA molecules (SSRMs), collectively short RNA molecules (SRMs),
- wherein the SSRMs and SARMs consist of 20-30 nucleotides;
- wherein said SARMs are complementary to, and can base pair with, a target RNA, which target RNA is transcribed from a gene that is silenced when said SRMs are present in a cell containing said gene, and said SSRMs correspond to the target RNA;
- and
- wherein said gene is endogenous to an organism selected from the group consisting of a plant, a mammal, an avian organism, a reptile, an insect, and a protozoan, or
- said target RNA is generated by a pathogen,
- whereby said gene is silenced.
3 more elements
A method of silencing a gene in cells which method comprises introducing into said cells at least one vector that, when introduced into said cells produces
- short antisense RNA molecules (SARMs) and isolated short sense RNA molecules (SSRMs), collectively short RNA molecules (SRMs),
- wherein the SSRMs and SARMs consist of 20-30 nucleotides;
- wherein said SARMs are complementary to, and can base pair with, a target RNA, which target RNA is transcribed from a gene that is silenced when said SRMs are present in a cell containing said gene, and said SSRMs correspond to the target RNA;
- and
- wherein said gene is endogenous to an organism selected from the group consisting of a plant, a mammal, an avian organism, a reptile, an insect, and a protozoan, or
- said target RNA is generated by a pathogen,
- whereby said gene is silenced.
4 more elements
Dependencies are inferred from the claim text.