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US 8,263,411 B2Granted
Capacitive morpholino diagnostics for analysis of nucleic acids
AssigneePolytechnic Institute of NYU
InventorsRastislav Levicky, Napoleon Tercero, Kang Wang, Ping Gong, Kenneth Shepard
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Abstract
A method to monitor the progress of hybridization between nucleic acid strands in solution and Morpholino strands immobilized on a solid support such as a working electrode in-situ, in real-time, and using label-free electrochemical measurements sensitive to hybridization-induced changes in the near-surface dielectric constant and charge organization.
Claims7
A method to monitor the progress of hybridization of nucleic acid strands in solution with Morpholino strands, comprising:
- immobilizing the Morpholino strands on a conductive solid support in the form of a working electrode to form a Morpholino probe;
- and
- conducting in-situ, in real-time, label-free electrochemical measurements of the surface capacitance of the Morpholino probe,
- wherein the label-free electrochemical measurements are sensitive to hybridization-induced changes in the near-surface dielectric constant and charge organization of the Morpholino probe and comprise measurements of interfacial impedance derived from alternating charging current signals generated in response to application of an alternating potential perturbation between the conductive solid support and the nucleic acid solution.
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Dependencies are inferred from the claim text.