Controllably degradable hydrogel for culturing cells to produce three-dimensionally organized cells
AssigneesMitsuru Akashi, Nof Corporation
InventorsMitsuru Akashi, Yoshiki Sawa, Michiya Matsusaki
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Abstract
To provide a new reductive-stimuli-responsive degradable gel that allows any control of decomposition of the three-dimensional base material for cell culture and production of a completely biological three-dimensional cellular structure consisting only of cells and cells-produced extracellular matrix and that allows safe recovery of the cellular structure produced. A stimuli-responsive hydrogel, characterized by being produced by crosslinking a water-soluble polymer with a compound having a disulfide bond in the molecular chain.
Claims11
A material for culturing cells to produce three-dimensionally organized cells, comprising a hydrogel produced by crosslinking a water-soluble poly(γ-glutamic acid) at a concentration of 4 to 9 wt % with a crosslinking compound having a disulfide bond in the molecular chain at an equivalence of functional group in the crosslinking compound of half or more of the equivalence of functional group in the water-soluble poly(γ-glutamic acid);
- wherein the hydrogel is capable of being controllably decomposed under a reductive atmosphere or in response to a reducing agent to separate three-dimensionally organized cells having a three-dimensional cellular structure consisting of cells and cell-produced extracellular matrix.
A method for producing three-dimensionally organized cells, comprising;
- culturing cells with a material for culturing to produce three-dimensionally organized cells comprising a hydrogel produced by crosslinking a water-soluble poly(γ-glutamic acid) at a concentration of 4 to 9 wt % with a crosslinking compound having a disulfide bond in the molecular chain at an equivalence of functional group in the crosslinking compound of half or more of the equivalence of functional group in the water-soluble poly(γ-glutamic acid),
- controllably decomposing the stimuli-responsive hydrogel with a reducing agent, and
- separating the three-dimensionally organized cells from the material, to separate the three-dimensionally organized cells having a three-dimensional cellular structure consisting of cells and cell-produced extracellular matrix.
A method for culturing cells to produce three-dimensionally organized cells having a three-dimensional cellular structure consisting of cells and cell-produced extracellular matrix, comprising:
- (a) culturing cells on a material comprising a hydrogel produced by crosslinking a water-soluble poly(γ-glutamic acid) at a concentration of 4 to 9% with a crosslinking compound having a disulfide bond in the molecular chain, at an equivalence of functional group in the crosslinking compound of half or more of the equivalence of functional group in the water-soluble poly(γ-glutamic acid), an
- (b) controllably decomposing the hydrogel under a reductive atmosphere or controllably decomposing the hydrogel by contacting it with a reducing agent to separate three-dimensionally organized cells having a three-dimensional cellular structure consisting of cells and cell-produced extracellular matrix.
Dependencies are inferred from the claim text.