Methods and compositions for stem cell self-renewal
AssigneeStowers Institute for Medical Research
InventorsJohn M. Perry, Linheng Li, Justin C. Grindley
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Abstract
The present invention relates to methods for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population.
Claims30
A method for ex vivo expansion of a population of hematopoietic stem cells obtained from a tissue selected from the group consisting of peripheral blood, cord blood, and bone marrow, the method comprising inhibiting PTEN and GSK-3β in the population of stem cells to expand the number of stem cells.
A method for ex vivo expansion of a hematopoietic stem cell population comprising inhibiting PTEN and GSK-3β in the hematopoietic stem cell population to expand the number of hematopoietic stem cells without significant differentiation of the hematopoietic stem cell population.
A method for ex vivo expansion of an hematopoietic stem cell (HSC) population obtained from a tissue selected from the group consisting of peripheral blood, cord blood, and bone marrow, the method comprising inhibiting PTEN and GSK-3β in the HSC population to expand the HSC population to a sufficient quantity while maintaining a multilineage differentiation potential in the HSC population, which is sufficient for subsequent transplantation into a patient in need thereof.
A method for ex vivo expansion of hematopoietic stem cells (HSCs) by at least 40-fold, the expanded HSCs being competent to reconstitute an HSC lineage upon transplantation into a mammalian patient in need thereof, the method comprising culturing a population of HSCs in a suitable culture medium comprising a PTEN inhibitor and a GSK-3β inhibitor.
Dependencies are inferred from the claim text.