US 8,263,396 B2 · Methods and compositions for the production of recombinant virus vectors

Abstract

A method for the production of a replication-deficient recombinant virus vector is disclosed. The replication-deficient recombinant virus vector has a recombinant virus genome with one or more defective viral genes. The method comprises infecting a host cell with a carrier virus having a carrier virus genome encoding one or more trans factors or variants thereof, incubating the infected host cell for a desired period of time, and isolating the replication-deficient recombinant virus vector. The carrier virus is a cytoplasmic virus that retains the carrier virus genome in the cytoplasm of the host cell. The host cell contains the recombinant viral genome and retains the recombinant viral genome in a nucleus of the host cell. Also disclosed is a carrier virus for the production of a replication-deficient recombinant virus vector.

Claims12

Dependent claims

An isolated cytoplasmic carrier virus, comprising:

a nucleotide sequence encoding one or more capsid proteins (VP1, VP2 and VP3) and one or more other structural proteins of an adeno-associated virus (AAV),
wherein said nucleotide sequence encoding one or more capsid proteins comprising one or more mutations at its original initiation codon from ATG to ACG or vice versa to assure a suitable ratio for production of a recombinant AAV vector, and wherein said nucleotide sequence is constructed to express said capsid and other structural proteins in the cytoplasmic compartment upon a host cell is infected with said carrier cytoplasmic virus.

Dependencies are inferred from the claim text.

Methods and compositions for the production of recombinant virus vectors

Life of this patent

Methods and compositions for the production of recombinant virus vectors

Life of this patent

Abstract

Claims12