Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors

A method for expressing a heterologous polypeptide in a cell, said method comprising:

• culturing a transfected cell clone to allow expression of the heterologous polypeptide, wherein cells of the transfected cell clone contain integrated into their genomes a transfected nucleic acid molecule comprising:
• a promoter functionally linked to an open reading frame encoding the heterologous polypeptide, said promoter comprising a human cytomegalovirus, a simian virus 40, an ubiquitin C, or an elongation factor one-alpha promoter, and
• a binding site for a p300/CBP protein, a P/CAF protein, or CBP, and wherein the cells of said transfected cell clone comprise said p300/CBP protein, P/CAF protein, or CBP,
• wherein the transfected nucleic acid molecule comprising the promoter functionally linked to an open reading frame encoding the heterologous polypeptide is flanked on one or both sides by a Stabilizing Anti-Repressor (STAR) molecule comprising a nucleic acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7.