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US 8,263,392 B2Granted
Methods and compositions related to continuous flow thermal gradient PCR
AssigneeUniversity of Utah Research Foundation
InventorsBruce Kent Gale, Niel Davenport Crews, Carl Thomas Wittwer
Life of this patent
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Abstract
Disclosed are compositions and a method for amplification and detection of nucleic acid sequences based on continuous flow thermal gradient PCR.
Claims53
A device for replicating nucleic acid, said device comprising:
- a microchannel extending from an inlet port to an outlet port;
- and a heater or heaters for producing a spatial temperature gradient, wherein the microchannel has a heating portion and a cooling portion, wherein each portion of the microchannel has a width, wherein the width of the heating portion is wider than the width of the cooling portion, and wherein the heater or heaters are located adjacent to the transition from the heating portion to the cooling portion.
A device for replicating a nucleic acid, said device comprising:
- a microchannel;
- two plates;
- and a heater;
- wherein the microchannel is sandwiched between the two plates and the heater is operable to form a spatial temperature gradient across the microchannel, the microchannel has a heating portion and a cooling portion, wherein each portion of the microchannel has a width, wherein the width of the heating portion is wider than the width of the cooling portion, and wherein the heater or heaters are located adjacent to the transition from the heating portion to the cooling portion.
1 more element
A device for replicating nucleic acid, said device comprising:
- a channel having a plurality of sections forming a continuous pattern;
- and a heater disposed along a centerline of the continuous pattern;
- wherein each section of the channel comprises a first portion and a second portion, the first portion of the channel being narrower than the second portion of the channel, wherein the first portion of the channel is a heating portion and the second portion of the channel is a cooling portion, and wherein the heater or heaters are located adjacent to the transition from the heating portion to the cooling portion.
A method of amplifying a nucleic acid, the method comprising the steps of:
- a) forming a steady state temperature gradient on a device comprising microchannels and a heater or heaters, wherein the microchannel has a heating portion and a cooling portion, wherein each portion of the microchannel has a width, wherein the width of the heating portion is wider than the width of the cooling portion, and wherein the heater or heaters are located adjacent to the transition from the heating portion to the cooling portion;
- and
- b) exposing a nucleic acid to the temperature gradient in a manner conducive for amplification;
- thereby amplifying a nucleic acid.
1 more element
Dependencies are inferred from the claim text.