US 8,263,387 B2Granted

Sheath flow devices and methods

AssigneeCynvenio Biosystems, Inc.

InventorsPaul Pagano, Yanting Zhang, Jiangrong Karen Qian, Hyongsok Tom Soh, Paul W. Dempsey, Andre′ De Fusco

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Abstract

The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products.

Claims50

Dependent claims

A sheath flow device comprising:

(a) a first laminar flow establishing surface upstream of a sheath flow plane area configured to establish a sheath laminar flow in the sheath flow plane area;
(b) a second laminar flow establishing surface parallel to the sheath flow plane area and upstream of a sample sheath flow plane area, wherein the second laminar flow establishing surface is configured to establish a sample laminar flow in the sheath flow plane area;
and
(c) a particle movement station configured to deflect a target species from the sample laminar flow and into the sheath laminar flow, wherein the particle movement station has a substantially rectangular interior space for bounding the sample laminar flow and the sheath laminar flow, wherein the interior space has first and second lateral dimensions transverse to the sample laminar flow, wherein the second lateral dimension is at least about 2 times larger than the first lateral dimension such that nonspecific binding is reduced along the larger of the first and second lateral dimensions.

1 more element

A fluidic separating device comprising:

(a) at least one sample inlet channel configured to provide a sample stream in the fluidic separating device;
(b) at least one sheath flow inlet channel configured to provide one or more fluid sheaths within the fluidic separating device and separating the sample stream from a surface of the device, thereby reducing nonspecific binding of components of the sample to the device;
and
(c) a sorting station fluidly coupled to the sample and sheath flow inlets and located along a path of the sample stream, wherein the sorting station has a substantially rectangular interior space for bounding the one or more fluid sheaths, wherein the interior space has first and second lateral dimensions transverse to the sample stream's direction of flow, wherein the second lateral dimension is at least about 2 times larger than the first lateral dimension such that nonspecific binding is reduced along the larger of the first and second lateral dimensions, and wherein during operation the sorting station has a magnetic field gradient effective to deflect and/or trap magnetic particles from the sample stream.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 12/813,285Filed 10 Jun 2010 · us provisional application
Priority: 10 Jun 2009
Published: US 2011/0003303 A16 Jan 2011
Granted: 11 Sep 2012
Term ends: Not estimatedThe source records don’t support an estimate.

Classification23 codes

G01N 33/54366
With an insoluble carrier for immobilising immunochemicals: apparatus specially adapted for solid-phase testing
B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/502776
Characterised by multiphase flow arrangements: specially adapted for focusing or laminating flows
B03C 1/01
Pretreatment specially adapted for magnetic separation: by addition of magnetic adjuvants