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US 8,263,382 B2Granted
Alpha-amylase mutants
AssigneeNovozymes A/S
InventorsAllan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Abstract
The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.
Claims20
A variant of a parent alpha-amylase, wherein the variant
- (a) comprises one or more substitutions selected from the group consisting of:
- L196R,K,D,E,F,Y;
- I201bulkier amino acid;
- Y203Q;
- I212R,K,D,E;
- L230H,K,I;
- A232G,H,F,S,V;
- V233D;
- K234L,E;
- and
- I236R,K,N,H,D,E;
- wherein SEQ ID No. 2 is used for position numbering;
- (b) has at least 90% homology with the amino acid sequence shown in SEQ ID NO:2, 4, 6, 38, 39, or 40;
- and
- (c) has alpha-amylase activity.
12 more elements
Dependencies are inferred from the claim text.